Name and Model (Year of Manufacture / Installation)

 Nikon AR1 (2013/2013)

General Information

High resolution and sensitive confocal system for live and fixed cell imaging. Suitable for photoactivation, photobleaching and FRET studies as well as multipoint and large area imaging.

Key Features

Ti-E inverted microscope is equipped with

• 405 nm diode laser, multiline Argon-laser (458 nm, 488 nm and 514 nm lines), 561 nm Sapphire laser and 642 nm diode laser.
• Two normal and two GaAsP PMT detectors for fluorescence and one PMT detector for transmitted light. Fluorescence detectors with emission filters (BP 450/50, 482/35, 515/30, 540/30, 595/50, (700/75) and LP 660).
• CFI Plan Apochromat Lambda 4X (N.A. 0.20, W.D. 2.0 mm), CFI Plan Apochromat Lambda 20X (N.A 0.75, WD 1 mm), CFI Plan Apochromat Lambda 40X (N.A 0.95, WD 0.25-0.16), CFI Plan Apo VC 60XH (N.A 1.4, WD 0.13, oil immersion) and CFI Plan Apo VC 60x (N.A 1.2, WD 0.31-0.28, water immersion) objectives.
• Nomarski DIC imaging possibility with 40x and 60x objectives.
• Galvanoscanner for high-resolution imaging and resonant scanner for high-speed imaging. Scanners can be used simultaneously (photoactivation, photobleaching and photoconversion with 405 nm laser and galvanoscanner, simultaneous imaging with resonant scanner).
• Motorized stage with the Piezo insert that allows fast and accurate multipoint imaging.
• Perfect Focus System to correct focus drift in time-lapse experiments.
• Humified stage-top CO2-incubator and large heated incubator (Okolab) for live cell work.
• NIS Elements confocal imaging software with Calcium&FRET plug-in and JOBS module.