Name and Model (Year of Manufacture / Installation)

Carl Zeiss Cell Observer HS (2009/2009)

General Information

High speed live cell imaging system with one-color TIRF.

Key Features

Inverted microscope equipped with

• Colibri LED light source (365, 455, 470, 505, 590, 625 nm) and XBO lamp.
• Zeiss Filter sets
  • 56HE eGFP/DsRed,
  • 59HE ECFP/EYFP,
  • 62 BFP/GFP/HcRed,
  • 60 CFP/YFP/HcRed,
  • 52 GFP (suitable for TIRF)
  • 64HE mCherry
  • 51 CFP
  • Two cameras: Zeiss AxioCam MRm (1388 x 1040 pix, pix size 6.45 µm) for high-resolution imaging and Rolera EM-C2TM (1004 x 1002 pixels, pix size 8 x 8 µm) for low light or high speed imaging.
  • Achroplan 20x (NA=0.45), Plan-Neofluar 40x (NA=0.6), Plan-Neofluar 63x (NA=1.2) water/glycerol immersion, Plan-Apochromat 63x (NA=1.4) Ph3 oil immersion and Plan-Apochromat 100x (NA=1.46) objectives.
  • DIC and phase contrast imaging possibility.
  • Large XL incubator with accurate CO2 and temperature feedback (Pecon).
  • Motorized stage for multipoint-imaging.
  • Definite focus system to correct focus drift.
  • TIRF (100x, NA=1.46, Plan-Apochromat objective, Filter set 52) with multiline Argon laser (458nm, 488nm and 514nm).
  • Aviovision Rel.4.8 software.